#### Tophat Mapping Report from the logs TophatMappingReport(){ ## todo replace source with simple R script as for bowtie-report echo ' devtools::source_url("http://dl.dropbox.com/u/113630701/rlibs/deepseq/ngs_tools.R") createMappingReport() ' | R -q --vanilla } #### Bowtie Mapping Report from the logs Bowtie2MappingReport(){ echo ' devtools::source_url("http://dl.dropbox.com/u/113630701/rlibs/base-commons.R") logSuffix=".logs" parseAlgnSummary <- function(alignSummary){ #alignSummary="./H2Az_Rep1_Lane1_Lib4454.bowtie.log" algnData <- readLines(alignSummary) data.frame( condition=trimEnd(basename(alignSummary), logSuffix), num_reads=as.numeric(str_split_fixed(algnData[3], " ", 2)[1]), unique_mappers=as.numeric(str_split_fixed(str_trim(algnData[6]), " ", 2)[1]), mapping_efficiency=as.numeric(str_replace(str_split_fixed(algnData[8], " ", 2)[1], "%", "")), multi_mappers=as.numeric(str_split_fixed(str_trim(algnData[7]), " ", 2)[1]) ) } mapStats <- ldply(list.files(".", logSuffix, full.names=TRUE, recursive=T), parseAlgnSummary, .progress="text") write.delim(mapStats, file="mapStats.txt") ggplot(melt(mapStats), aes(condition, value)) + geom_bar(stat="identity") +facet_wrap(~variable, scales="free") + ggtitle("mapping summary") + scale_y_continuous(labels=comma) + theme(axis.text.x=element_text(angle=90, hjust=0)) ggsave2(w=10, h=10, p="mapstats") ggplot(mapStats, aes(condition, mapping_efficiency)) + geom_bar(stat="identity") +coord_flip() + ylim(0,100) + ggtitle("mapping efficiency") ggsave2(p="mapstats") ggplot(mapStats, aes(condition, num_reads)) + geom_bar(stat="identity") + coord_flip() + ggtitle("read counts") ggsave2(p="mapstats") ggplot(mapStats, aes(condition, unique_mappers)) + geom_bar(stat="identity") + coord_flip() + ggtitle("unique alignment") + scale_fill_discrete() ggsave2(p="mapstats") ' | R --vanilla } export -f Bowtie2MappingReport