error in the plotDispEsts()

https://git.mpi-cbg.de/bioinfo/ngs_tools/blob/master/dge_workflow/featcounts_deseq.R#L143

Never saw this one. How does your count-matrix look like?

Here is the countMatrix. seems allrite to me.

dim(countMatrix)

[1] 12010 18

&colData

where is the complete matrix?

projects/bioinfo/lakshman/projects/sarita_flyome/ProjectID787/alignments/star_counts_matrix.txt

star_counts_matrix.txt

I hope I solved this by calling DESeq function before the plotDispEsts in line 132 ans I also loaded the readr library for write_tsv which gave me an error

https://git.mpi-cbg.de/bioinfo/ngs_tools/blob/master/dge_workflow/featcounts_deseq.R#L132

That's what I did until Mathias told me recently (see #f047009a ) that it's not necessary. Next time I won't blindly believe him.

Status changed to closed