Commit ebb14943 authored by Holger Brandl's avatar Holger Brandl

updated limma instructions

parent 873e2471
......@@ -29,4 +29,10 @@ RNA-Seq with DESeq2
Differential expression with limma
----------------------------------
Prepare design, contrasts and expression/abundance matrix as for DESeq2 workflow and run
Prepare `design`, `contrasts` and `expression/abundance matrix` as for DESeq2 workflow and run
```bash
rend.R --toc ${NGS_TOOLS}/dge_workflow/limma/dge_limma.R --contrasts example_contrasts.txt ../inten_matrix_acc.txt ../exp_design.txt
```
TO provide custom gene info for non-ensembl ids, provivde `--gene_info <tsv_with_gene_id_as_first_column>` argument.
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