... | ... | @@ -50,7 +50,7 @@ PeakStrainer groups peaks across multiple scans, but the peak m/z may not be exa |
|
|
|
|
|
The size of the bin, in other words the range of m/z values that are considered the same, is variable throughout the full m/z range; usually smaller for low m/z values and larger for high m/z values.
|
|
|
|
|
|
There are two approaches to determine the bin size at a certain m/z value. One is to take the measured resolution of the peaks at full width half maximum FWHM and calculate the trend function trough the full m/z range, we then use the function to determine the bin size. The other approach is to provide personalized function parameters for MS1 and MS2 respectvely, this allows for more fine grained handling of the data.
|
|
|
There are two approaches to determine the bin size at a certain m/z value. One is to take the measured resolution of the peaks at full width half maximum FWHM and calculate the trend function troughout the full m/z range, we then use the function to determine the bin size. The other approach is to provide personalized function parameters for MS1 and MS2 respectively, this allows for more fine grained handling of the data.
|
|
|
|
|
|
Bins are created based on peaks m/z values, therefore some bins might overlap with one another, you can choose to merge the overlapping bins into one larger bin, that may have a higher peak count, therefore a higher repetition rate.
|
|
|
|
... | ... | @@ -60,15 +60,13 @@ Bins are created based on peaks m/z values, therefore some bins might overlap wi |
|
|
|
|
|
Repetition Rate
|
|
|
----------
|
|
|
Once we have removed signals that do not repeat, in the prefilter stage, and we have grouped signals that are the same in the binning stage, now we can discard signals that are not repeated often enough throughout scans. The threshold for how often the signal must repeat throughout scans is given as a percentage of all the scans, ie Minimum repetition rate.
|
|
|
Once we have removed signals that do not repeat, in the prefilter stage, and we have grouped signals that are the same in the binning stage, now we can discard signals that are not repeated often enough throughout scans. The threshold for how often the signal must repeat throughout scans is given as a percentage of all the maximum repetition rate, called the Minimum repetition rate.
|
|
|
|
|
|
![Capture7](/uploads/83cececcd09aaafc540fa350b0334896/Capture7.PNG)
|
|
|
|
|
|
|
|
|
|
|
|
Status Page
|
|
|
----------
|
|
|
When the finish button is pressed you will go to the status page, here some text information is presented to show how the progress of the process.
|
|
|
When the finish button is pressed you will go to the status page, here text information is presented to show the progress of the process.
|
|
|
|
|
|
If a problem occurs it will be reported here.
|
|
|
|
... | ... | @@ -76,7 +74,7 @@ If a problem occurs it will be reported here. |
|
|
|
|
|
Finish
|
|
|
----------
|
|
|
Once the files have been processed, new *.mzXML files are created and the [Reorder Scans](Reorderscans) application may start, you can also start [Reorder Scans](Reorderscans) by clisking on the *Reorder Scans *button in the start screen
|
|
|
Once the files have been processed, new *.mzXML files are created and the [Reorder Scans](Reorderscans) application may start, you can also start [Reorder Scans](Reorderscans) by clisking on the *Reorder Scans* button in the start screen
|
|
|
|
|
|
|
|
|
|
... | ... | |