8. Continue until all 30s-long submovies of one TLM are analyzed and save all measured angles of actomyosin signals of one TLM in an Excel file.
9. Sum up all measured angles over the relevant number of TLMs (dependent on the experiment)
10. Plot the percentage of the direction of analyzed actomyosin signals as a histogram or as a rose diagram with a range from 0° to 360° with a preferred software.
10. Plot the percentage of the direction of analyzed actomyosin signals as a rose diagram with a range from 0° to 360° with a preferred software.
* NOTE: To obtain statistically significant results, 5-10 independent egg chambers of any egg chamber stage are recommended to be analysed.
*Adapted from Figure 1:*[Viktorinova *et al.*, 2017](https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007107)
