README.md 1.37 KB
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# Compartmentalised RNA catalysis in membrane - free coacervate protocells
## Codes 
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### For Fluorescence Recovery after Photobleaching
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**Fiji plugin**
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The file 'Frap_in_Fiji.ijm' had to be located in the plugins folder of the Fiji distribution.
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*The plugin asked for the folder containing the microscope images.
*Images were opened by Fiji and displayed individually including saved ROIs.
*ROIs could be adjusted followed by user confirmation.
*Date were extracted and saved in the 'Analysis' subfolder
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**Matlab scripts**
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The 'BuildAndFit.m' script
* loading data
* normalisation
* call fit function 'FitFRAP.m"
* call plotting function "FRAPplot.m'
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The 'FitFRAP.m' script
* input from 'BuildAndFit.m'
* fit data to equation 5 in the manuscript
* returns optimised parameters
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The 'FRAPplot.m' script
* needs parameters from FitFRAP
* calculate diffusion constants
* plot results
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### For Fitting of Kinetics
The scripts 'SaturFitMonoExp.m' and 'SaturFitBiExp.m' 
* input is a kinetic data array and a vector containing corresponding timepoints
* data fitting to either single exponential ('SaturFitMonoExp.m') or biexponential ('SaturFitBiExp.m') function
* returns optimised parameters and fit function
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## Data availability
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All Data could be downloaded upon request at corresponding author^± via this [link (coming soon)](https://www.google.com).  
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[T-Y. Dora Tang](mailto:tang@mpi-cbg.de) 
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