Skip to content

GitLab

Closed
Created by domingue@domingueMaintainer

dge_workflow: echoing R code in to Rscript not loading personal library packages

The function dge_star_counts2matrix, located in dge_utils stops with the error:

devtools::source_url("https://git.mpi-cbg.de/bioinfo/datautils/raw/v1.40/R/core_commons.R")
Error in loadNamespace(name) : there is no package called ‘devtools’
Calls: :: ... tryCatch -> tryCatchList -> tryCatchOne -> <Anonymous>
Execution halted

I tracked it down and this occurs because of the flag "--vanilla" in:

| R --no-save --no-restore --no-site-file -q. This flag keeps the R environment "clean" but that also means R packages installed in personal libraries are not loaded - thus the error despite devtools being installed.

There are two (three) solutions for this:

  1. Replace --vanilla flag with --no-save --no-restore --no-site-file since vanilla is in fact a wrapper for --no-save, --no-restore, --no-site-file, --no-init-file and --no-environ. Removing the flags causing the issue will work (I tested it).
  2. Instead of echoing the Rcode (echo '[some code]' | R --no-save --no-restore --no-site-file -q) we could the approach Rscript - <<"EOF" [some code] EOF. This was also tested and also works, but I have not looked into unintended consequences (loading of hidden R files for instance).
  3. More of a long term solution, and probably not feasible, keep these R snippets in their separate .R files, or as functions, a call them with Rscript some_function.R.

For the time being I would suggest either of 1. and 2. @herseman If you have any preference let me know so that I make the PR.

A consequence of this bug, and that was how I found it, is that star_align.kts will not produce the count matrix table and, afaik, finish successfully with a reported warning.