star_align.sh

#!/usr/bin/env bash


#https://www.biostars.org/p/91020/

usage='
Use star to align fastq files against a genome
Usage: star_align.sh <igenome> <fastq_files>...

Options:
-c        Cache results
'

#eval $(echo "$usage" | ~/bin/docopts/docopts -h - -A dopts : "$@")
#echo "$usage" | ~/bin/docopts/docopts -h -  : "$@"
#echo "$usage" | ~/bin/docopts/docopts -h - : "hallo dfds"
#eval $(echo  $usage | ~/bin/docopts/docopts -h - : "$@")
#eval(exit 64)
eval "$(echo  "$usage" | ~/bin/docopts/docopts -h - : "$@")"

# v0.7 style
#eval $(echo  "$usage" | /home/brandl/bin/docopts_v0.7/docopts "hallo")

#eval "$(echo "$usage" | ~/bin/docopts/docopts -h -  : /projects/bioinfo/igenomes/Mus_musculus/Ensembl/GRCm38 /projects/bioinfo/holger/projects/florio_11b_2nd_batch/lanereps_pooled/arhgap11b_1.fastq.gz fastq2)"


## extract all configuration parameters
fastqFiles=${fastq_files[@]}
export star_index="${igenome}/Sequence/StarIndex"
export gtfFile="$igenome/Annotation/Genes/genes.gtf"

#echo $fastqFiles
#head $gtfFile

if [ ! -f $gtfFile ]; then
    >&2 echo "gtf '$gtfFile' does not exis"; exit 1;
fi


if [ -z "$(which STAR)" ]; then
    >&2 echo "no STAR binary in PATH"; exit 1;
fi

## basic usage tutorial
#http://www.homolog.us/blogs/blog/2012/11/02/star-really-kick-ass-rna-seq-aligner/

## build index if not present
if [ ! -f "${star_index}/SA" ]; then
    echo "Missing STAR for ${star_index}/SA; use 'dge_create_star_index ${igenome}' to create it"; exit 1;
#    dge_create_star_index ${igenome}'
fi


echo "running STAR using igenome '${igenome}' for the following files"


#fastqFiles=$(ls $baseDir/treps_pooled/*fastq.gz)

for fastqFile in $fastqFiles ; do
    echo "submitting STAR job for $fastqFile"

    # DEBUG fastqFile=/projects/bioinfo/holger/projects/florio_11b_2nd_batch/lanereps_pooled/arhgap11b_1.fastq.gz
    fastqBaseName=$(basename ${fastqFile%%.fastq.gz})
    outputdir=$fastqBaseName

    ## uniquely mapping reads only:   http:/seqanswers.com/forums/showthread.php?s=93da11109ae12a773a128a637d69defe&t=3464
    ###     tophat -p6  -G $gtfFile -g1 -o test $bowtie_gindex $fastqFile

    ## params:
    # --outSAMstrandField intronMotif is required for cuffdiff compatiblity (xs flag)
    # --outFilterIntronMotifs RemoveNoncanonicalUnannotated --outFilterType BySJout to get rid of artifical junctions
    # --quantMode GeneCounts see https://groups.google.com/forum/#!searchin/rna-star/GeneCounts/rna-star/gZRJx3ElRNo/p5FjBYKuY00J

    mysub "${project}__star__${fastqBaseName}" "
    STAR --genomeDir $star_index --readFilesIn $fastqFile --runThreadN 6 --readFilesCommand zcat --outFileNamePrefix ${fastqBaseName}. --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --sjdbGTFfile $gtfFile --outFilterIntronMotifs RemoveNoncanonicalUnannotated --outFilterType BySJout --quantMode GeneCounts
    mv ${fastqBaseName}.Aligned.sortedByCoord.out.bam ${fastqBaseName}.bam
    samtools index ${fastqBaseName}.bam
    " -n 5 -R span[hosts=1] -q medium | joblist .starjobs
done

wait4jobs .starjobs


rm -rf *STARgenome *.Log.progress.out _STARtmp *.SJ.out.tab *Log.out
#ziprm star_outlogs *Log.out

dge_bam_correlate . &


## estimate expresssion with http://bioinf.wehi.edu.au/featureCounts/
##Summarize multiple datasets at the same time:
#featureCounts -t exon -g gene_id -a annotation.gtf -o counts.txt library1.bam library2.bam library3.bam

#bamFile=control_3.bam
#featureCounts -t exon -g gene_id -a ${gtfFile} -o feature_counts.txt ${bamFile} -T 5
mysub "${project}__feature_counts" "featureCounts -t exon -g gene_id -a ${gtfFile} -o gene_counts.txt $(ls *bam) -T 5" -q medium | blockScript

## create tophat mapping report
spin.R ${NGS_TOOLS}/dge_workflow/star_qc.R .

mailme "${project}: STAR done in $(pwd)"