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bioinfo
ngs_tools
Commits
54013ebe
Commit
54013ebe
authored
9 years ago
by
Holger Brandl
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use short queue for star
parent
f155bd8e
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dge_workflow/star_align.sh
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54013ebe
...
@@ -52,6 +52,7 @@ fi
...
@@ -52,6 +52,7 @@ fi
## build index if not present
## build index if not present
if
[
!
-f
"
${
star_index
}
/SA"
]
;
then
if
[
!
-f
"
${
star_index
}
/SA"
]
;
then
chmod
-R
+w
${
star_index
}
mailme
"
${
project
}
: creating STAR index for
$igenome
"
mailme
"
${
project
}
: creating STAR index for
$igenome
"
mkdir
${
star_index
}
mkdir
${
star_index
}
...
@@ -87,7 +88,7 @@ for fastqFile in $fastqFiles ; do
...
@@ -87,7 +88,7 @@ for fastqFile in $fastqFiles ; do
STAR --genomeDir
$star_index
--readFilesIn
$fastqFile
--runThreadN 6 --readFilesCommand zcat --outFileNamePrefix
${
fastqBaseName
}
. --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --sjdbGTFfile
$gtfFile
STAR --genomeDir
$star_index
--readFilesIn
$fastqFile
--runThreadN 6 --readFilesCommand zcat --outFileNamePrefix
${
fastqBaseName
}
. --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --sjdbGTFfile
$gtfFile
mv
${
fastqBaseName
}
.Aligned.sortedByCoord.out.bam
${
fastqBaseName
}
.bam
mv
${
fastqBaseName
}
.Aligned.sortedByCoord.out.bam
${
fastqBaseName
}
.bam
samtools index
${
fastqBaseName
}
.bam
samtools index
${
fastqBaseName
}
.bam
"
-n
5
-R
span[hosts
=
1]
-q
medium
| joblist .tophatjobs
"
-n
5
-R
span[hosts
=
1]
-q
short
| joblist .tophatjobs
done
done
wait4jobs .tophatjobs
wait4jobs .tophatjobs
...
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